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Lastly, in order to quantitatively compare the binding of these mutants to the 60S subunit, we measured the affinities of these mutants to the 60S subunit using Bio-layer interferometry (BLI) (Figs. 3E and S2).
To confirm the role of the PTB domain in tensin2 binding, we first tested the binding of these mutants with tensin2.
The binding of these mutants to the Pikp-HMA was tested in yeast and in vitro.
Our results indicate still a very weak binding of these mutants with PTP1B-D181A mutant.
Indeed, membrane binding of these mutants was decreased compared to SUMO-2 (Fig. 3f).
To test the binding of these mutants to β-catenin in vivo, we introduced them into full-length FLAG-tagged BCL9, and also generated the corresponding mutants in BCL9-2 (L408FigL411K; Fig. 1A), and co-expressed these with HA-tagged β-catenin in HEK 293T cells.
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The human respiratory tissue binding of these mutant H2 HAs was in agreement with their observed glycan array binding (Figure 5B, 5D, 5F).
We then studied the catalytic activities and DNA binding of these mutant proteins as well as some important functional protein interactions.
It has previously been shown that the TKB domain can bind to growth factor receptors and it will be important to determine the cross-binding of these mutants to MET and or EGFR.
The effect of bevirimat binding to these mutants likely promoted, rather than disrupted, virion maturation.
Binding analysis with P. xylostella BBMV, were reported only for E129K and D136N mutants, revealing no effects on binding of these two mutants, and suggesting that loss of binding was not the reason for the loss of toxicity in these Cry1Aa mutants [25].
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binding of these co-factors
binding of these ligands
binding of these effectors
binding of these structures
binding of these components
binding of these proteins
binding of these flavonoids
binding of these heterodimers
binding of these NPs
binding of these RBPs
binding of these peptides
binding of these SmTK6
binding of these drugs
binding of these complexes
binding of these substances
binding of these agents
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