Sentence examples for binding of the transposase from inspiring English sources

This transposition mechanism involves the binding of the transposase to the element TIRs and the excision of the element generating a DSB at the donor site followed by the integration of the element into a different chromosomal site.

A consideration of the conserved and unique aspects of Ds footprints in maize [27], [31], [32], Arabidopsis [23; this work] and yeast [4], [21] suggest that binding of the transposase molecules at Ds TIRs may be sufficient for accurate excision of Ds but that the mechanism of repair of broken ends at the donor site is dependent on host factors.

In vitro experiments revealed that a 16% or more difference in the TIRs can preclude the binding of the transposase [ 13].

While we did not attempt to detect the binding of the transposase to DNA by an electrophoretic mobility shift assay, we analyzed complex formation by analytical size-exclusion chromatography.

In this idealized situation, synapsis of the transposon ends is simply a product of the sequential binding of the transposase dimer to sites at opposite ends of the element.

A TIR IS element carries a pair of (partially conserved) inverted repeats at the two termini of an IS for cleavage and binding of the transposase, as shown in Figure 1 (a), while a non-TIR IS element) does not harbor significant signals around its termini.

The steady-state rate of transposition was reduced in proportion to the binding affinity of the transposase.

A and B represent the identical inverted repeat sequences flanking the transposon, which are the specific binding sites of the transposase.

Alignment of the DNA-binding domains of the transposases revealed a distinction between Trem elements, which may be functionally connected to the TIRs of these elements.

When high-affinity transposase binds to the Ds TIRs, binding of further transposase molecules to nearby cryptic sites may be facilitated.

It was suggested that this direct repetition could be the recognition and binding of the HTH and CENPB transposase domains [ 37].