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Moreover, we were able to reproduce the structural changes that occur upon the binding of the third Mg2+ to the active site of the protein.
All the results illustrate that binding of the third strand in either of the two orientations 5′5′3′ (JT2T4) or 5′3′3′ (JT1T3) stabilizes the underlying double-helical four-way junction and its triple-helical arms.
However, it seems more likely that binding of the third Ca2+ ion might lead to a gain of function rather than to a complete loss of function.
Note that this serine residue of loop 3 is not generally conserved in synaptotagmin C2B domains (Figure 2A) and hence binding of the third Ca2+ ion is an unusual feature of the synaptotagmin-7 C2B domain compared to other isoforms.
We showed that higher NaCl concentrations suppress binding of the third DBD molecule to DNA.
Based on previous reports, binding of the third Rev molecule (the second Rev dimer) and beyond requires higher oligomerization capability of the protein (Daugherty et al., 2008).
Similar(52)
If the compounds bind the immobilized protein, the binding of the second protein will be reduced; otherwise, the binding will be largely retained.
Upon binding of the fourth inhibitor the dimer interface, however, becomes partially disordered.
In the oxFRPcc case, this could explain the low efficiency of binding of the second OCP, unless this stoichiometry is fixed by chemical crosslinking (Fig. 4).
In this system, the binding of the first guest (1 2 saccharide/boronic acid complex) suppresses the rotational freedom of the two porphyrin planes, which facilitates the binding of the second guest.
Substrate activation can result from an allosteric effect due to binding of the second substrate molecule on the PAS.
More suggestions(14)
binding of the fifth
binding of the three
binding of the radiolabelled
binding of the active
binding of the targeted
binding of the immunoconjugate
binding of the secondary
binding of the polyclonal
binding of the hybrid
binding of the former
binding of the different
binding of the non
binding of the same
binding of the second
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