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The sole exception (R223A) can be attributed to weaker binding of the ternary complex to the ribosome, resulting from disruption of a contact that forms between them.
The biotin moiety on the capture probe mediates binding of the ternary complex (target/C-probe/capture probe) to streptavidin-coated beads.
As shown in Figure 7(a), EF-Tu promoted binding of the ternary complex to the A-site of approximately 50% of the ribosomes within 15 min.
As would be expected from the structure, we could see good binding of eEF2 but no binding of the ternary complex of eEF1A to the CrPV-IRES-ribosome complex.
As the concentration of EF-Tu present in the reaction was increased, the binding of the ternary complex to the ribosome increased), and this linear relationship shows the requirement for EF-Tu.
To see whether translocation of the IRES would allow binding of the ternary complex to the ribosome, the CrPV-IRES-ribosome complex was first incubated with eEF2 in the presence of GTP to facilitate translocation and pelleted through a sucrose cushion as above to remove GTP and excess eEF2.
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We next mapped the regions of ZNF198 required for binding to the ternary LCH complex or HDAC1-SUMO2.
The fact that we could measure the enthalpies of each individual process independently and accurately allowed us to tackle the binding analysis of the ternary E2C−DBS−E2C complex by ITC.
Edeine does not inhibit binding of the eIF2/GTP/Met-tRNAi ternary complex to the 40S ribosomal subunit, nor Met-tRNAi/40S complex scanning, but there is a complete failure of AUG codon recognition, so that scanning continues past all AUG codons, and, probably as a secondary consequence, there is no ribosomal subunit joining [24] [25].
We used purified recombinant proteins and RNAs to perform in vitro experiments that compare binding in the ternary complex system (RNA/SNF/U2A′) with bimolecular binding of SNF/U2A′ and SNF/RNA complexes.
A new method of identifying sublattice substitution of ternary additions in NiAl was proposed by comparison of the binding energies per atom of the ternary and the binary clusters involving the fourth nearest neighbours.
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