Sentence examples for binding of the single from inspiring English sources

Moreover, conditions of lower molecular crowding favour binding of the single strand binding protein RPA [65] to ssDNA, thus preventing DNA hybridization.

This effect might not necessary rely on actin bundling since binding of the single ABDs of L-plastin and its closely related T-plastin isoform, were sufficient for decreasing actin depolymerization in vitro [20], [46].

The binding of the single or double mutants containing the G228S mutation on ciliated cells was abolished by treatment with α2-3-specific NA (compare Figure 5C and 5D, left panel, open triangles, with Figure 5C and 5D, middle panel).

Binding of the single chain phage antibodies to the cells was visualized using a biotinylated anti-M13 antibody (Fitzgerald) followed by streptavidin-phycoerythrin conjugate (Caltag).

The HepA-related protein (HARP/SMARCAL1) is an ATP-dependent annealing helicase that is capable of rewinding DNA structures that are stably unwound due to binding of the single-stranded DNA (ssDNA -binding protein Replication Protein A (RPA).

Each of the double substitutions also retains the ligand binding features of the single mutations it contains.

To isolate a stable complex containing both full-length proteins, and to analyze binding activity of the single mutations of FUS to Pur-alpha, we immunoprecipitated FUS from HeLa cells co-expressing each Flag-FUS mutant with HA-Pur-alpha.

Here, we examine the natural DNA-binding sites of the single Drosophila Stat and show that this is not the case.

For instance, the variant both restricts the DNase I footprint in the region [ 9] and reduces the binding of the mitochondrial single-strand DNA binding protein (mtSSB) [ 37].

Briefly, the protocol comprises the following steps: bisulfite-mediated conversion of unmethylated cytosines, binding of the converted single-stranded DNA to the membrane of an EpiTect spin column, desulfonation of DNA and washing and elution of the pure, converted DNA from the membrane.

The two-tissue compartment model provided a direct estimate of the binding potentials of [N-methyl-11C]mirtazapine in brain regions, while binding potentials of the single-tissue compartment model and the graphical plasma input model were estimated indirectly from ratios of distribution volumes in brain regions.