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To examine whether there is preferential binding of ligand to the four different CR domains, we examined binding of the simplest ligand, Nα-acetyl-lysine methyl ester, to each domain.
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Because there are only a small number of relevant binding sites (in the simplest case, just one), the occupancy of these sites must fluctuate, and this random switching is an additional source of noise.
Depending on the lectins, some discrepancies were noticed in the binding of simple sugars to the carbohydrate-binding site.
The spatial organization of the amino acid triad D-N-Q forming the carbohydrate-binding site of EULs, accounts for the promiscuity in the binding of simple sugars and oligosaccharides to the β-trefoil domains of E. europaeus lectins.
This work also addresses the problem of TSV allocation and optimization, but overcomes one of the critical limitations of the previous works, which is the unawareness or no exploitation of the possibility of TSV resource sharing, previously merely resorting to a simple binding of the data transfers to TSVs.
Representing one of the simplest key-binding approaches the fuzzy commitment scheme [15] has been successfully applied to iris [32] (and other biometrics).
Additional stacking interactions between the sugar rings and the aromatic residues F118 and W136, complete the binding of simple sugars and disaccharides to the carbohydrate-binding site.
The stipulatio was the most important form of verbal contract, for it established a form in which any agreement (provided it was lawful and possible) could be made binding by the simple method of reducing it to question and answer: "Do you promise to pay me 10,000 sesterces?" "I promise".
We found that binding of our simple amino acid ligands to the four CR domains gave saturable perturbation of CR fluorescence, as expected from a specific binding interaction.
It has been argued that such a "fly-casting" mechanism [ 97] has a much higher efficiency in the search for binding partners than the simple diffusion of a compact, folded protein.
Glycosylation, not to be confused with the non-enzymatic binding of simple sugars as occurs with hemoglobin A1c in patients with diabetes mellitus [ 40], is an enzymatic modification whereby an oligo or polysaccharide moiety is attached to a protein or lipid.
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