Sentence examples for binding of the secondary from inspiring English sources

Any resulting immunofluorescence would suggest unspecific binding of the secondary antibodies.

The plate was incubated 1 hour at 37°C to allow the binding of the secondary antibody.

This may lead to a different structure of the 30S subunit since binding of primary binding proteins affects binding of the secondary and tertiary binding proteins [116].

The green channel intensity (G) was not affected by the binding of the secondary antibody (data not shown), and was entirely the result of autofluorescence (GAF).

Unspecific signals resulting from direct binding of the secondary antibody to the peptides were subtracted from the raw signal in every experiment to obtain sera-specific net reactivities.

Tissue sections in which primary or secondary antibodies were omitted were kept as a control to detect background due to autofluorescence and/or none specific binding of the secondary antibodies.

To confirm the influence of CIT binding on the secondary structure of HSA, CD spectra are performed in the presence of different concentrations of CIT.

The effect of ANS binding on the secondary structures of PDC-109 was analyzed by CD spectroscopy using a Jasco J-810 spectropolarimeter fitted with a thermostatted cell holder and interfaced to a thermostatic waterbath.

The premise of this strategy, "collaborative enhancement", is that the initial binding event can lead to an increased binding event of the secondary targeting agent.

As we did not evaluate the binding affinities of the secondary reagents we were not able to draw meaningful conclusions regarding a T1 or T2 bias based upon the ratio of IgG1 to IgG2 titres [ 15].

A check of the primer regions found multiple mismatches in predicted primer binding sites of the secondary hit for three of the markers; these were assumed to have amplified as single-copy.