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We additionally performed FACS-analysis with EGFP-fused CROPs to investigate binding of the repetitive sequences to different host cells.
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In an effort to improve the specificity of target binding to the microarray for BAC hybridization, we employed a Cot-1 DNA protocol to reduce the binding of these repetitive elements (Table 4).
It is therefore likely that this colocalisation is due to nonspecific binding, and the repetitive nature of the avian W chromosome.
The binding of STAT1 within repetitive sequences of the LTR class might relate to the description of functional GAS sites in retroviruses [44].
In array-CGH, test and reference DNAs are labelled with different fluorophores (for example, Cy5 and Cy3), and then simultaneously hybridised onto arrays in the presence of Cot-1 DNA to reduce the binding of repetitive sequences.
Because of the repetitive nature of the DNA binding domain, the assembly of customized TALEs by direct synthesis or traditional cloning is expensive and technically challenging.
Deivanayagam, C. C. et al. Novel fold and assembly of the repetitive B region of the Staphylococcus aureus collagen-binding surface protein.
The probe was allowed to pre-hybridise for half an hour at 37°C to reduce unspecific binding of short or repetitive DNA pieces.
Because of the repetitive structure of terminology system rubrics, the word alignment differences were also repetitive.
The observation of the binding of activated STAT1 protein to a specific repetitive element bolsters similar reports concerning p53 and other TFs, and strengthens the notion of an involvement of repeats in gene regulation.
Several groups have considered the use of recombinant antigens based on the repetitive binding domains (RBD) located at the C terminus of both toxins -.
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