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Within each Puf repeat, three conserved amino acid side chains are typically responsible for modular binding of the repeat to a single RNA base using hydrogen bonds, van der Waals, and base stacking interactions [ 12].
In contrast, almost all of the hits isolated only once recognized the anti-ADP3 antibodies with far lower affinities that more closely resembled the binding of the repeat hits to the control antibodies.
Our RNA pulldown screen for binding of the repeat expansion identified multiple messenger RNA export adapters including ALYREF (Stutz et al., 2000), SRSF1, SFRS3 and SFRS7 (Huang et al., 2003; Hargous et al., 2006; Tintaru et al., 2007).
Two polymorphic aa residues at positions 12 and 13 form the repeat-variable diresidue (RVD), where the residue 13 is responsible for preferential binding of the repeat module to a single specific nucleotide in the major groove of target DNA sequence (summarized in [ 10]).
While the main point of this study was to evaluate the wisdom of focusing solely on repeat hits in postscreening efforts, the analysis of the determinants of binding of the repeat hits to the anti-ADP3 antibodies was also carried out and provided interesting data.
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We therefore performed NMR spectroscopy and ITC experiments on the binding of this repeat with the GPCR-derived FBM peptides.
The amino acid combination at those four positions thus presumably defines the functional binding properties of the repeat unit.
Indeed, the affinity of the peptoids 5– 8 for the anti-ADP3 antibodies more closely resembled the binding of the four repeat hits to the control antibodies (compare Figure 4B and 4D).
The chloroplast genome is a dynamic structure in which the collinear binding of the inverted repeat regions likely leads to a handle-like structure isolating the LSC and SSC regions in separate domains and serving as a basis for occasional recombinational events that result in an inversion of the SSC region [ 23].
The interface residues at sites 1, 2, and 3 for the binding of the ANK repeats to AnkR_AS are labeled with '1', '2', and '3', respectively.
Expression of COR genes under cold stress in Arabidopsis is regulated through the binding of the C-repeat binding factor (Cbf) gene family to the cis-regulatory element DRE/CRT present in the promoter region of COR genes [ 2, 19].
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binding of the outbreak
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