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The hydrodynamic properties of StyA isozymes were found to be modulated by the binding of the (reduced) FAD cofactor.
No significant differences were seen between the binding of the reduced and control antibody preparations.
This analysis revealed several structural features specific of plant GDH4: (i) the lack of a structure called "antenna"; (ii) the NAD(P -binding motif GAGNVA; and (iii) a second P -bindingoenzymotifndinGAGNVAf GVLTGKG together with four residues involved in the binding of the reduced form of Nand.
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(A ) The binding affinity of the reduced and alkylated PfRh5 to basigin measured by SPR.
The Salmonella thioredoxin homolog possibly acts by reducing free Cu ions through regulating the binding capacity of the reduced form of thioredoxin to Cu ions [ 40].
The O2 reaction and NO binding of the fully reduced Tt ba3 G232V mutant were investigated by mixing the mutant with the O2 or NO carrier and photolyzing the respective complex with a 355 nm laser pulse.
To deduce the structural basis of the reduced binding affinity, we solved the crystal structure of vcCNT bound to zebularine.
However, this inhibition does not reflect direct inactivation of QSOX or PDI, but avid binding of MVAs to the reduced unfolded protein substrates themselves.
By contrast, at inhibitory synapses the binding of neurotransmitters reduces the chances of the postsynaptic cell firing.
In each case, prior binding of nucleotide reduced the binding affinity of drug.
We furthermore applied a polyethylenglycol (PEG) linker for coupling of the RGD-ligand, which brings several advantages, such as a reduced non-specific binding of the avidin and reduced immunogenicity [ 19].
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