Sentence examples for binding of the probe from inspiring English sources
Indeed the antibodies against Mxi1 interfere with the DNA binding, thus indicating that they bind the DNA binding domain of Mxi1 and competition for the binding of the probe.
The binding of the probe to all the three deformed DNA duplexes is accompanied by an increase in the thermal melting stability of the deformed DNAs.
Strong binding of the probe to the organoclays resulted in a potential shift of ca. 0.1 V of the voltammetric signals characteristic of the Fe CN 63−/4− couple in the anodic direction.
Inappreciable change in the CD spectrum of ctDNA with the addition of DSDP suggests that binding of the probe with the DNA does not lead to a significant modification in the DNA conformation.
Sequence-specific binding of the probe was further verified by successfully out-competing the hybridization with 10 × molar excess unlabeled probe and failure of a labeled sense probe to hybridize.
After interaction events, the fluorescence signal is activated through specific binding of the probe with target protein in cell, causing the conformation alteration and forcing the separation of fluorophore from the quencher.
We filtered out the noise from the background by excluding the dots with the weakest intensity due to nonspecific binding of the probes (typically consisting of less than 5% of all the dots).
The high affinity binding of the probes QE and bivQ to the CCK2R in combination with internalization and hence signal amplification is an important prerequisite for low dosage for the prospective detection of CCK2R-expressing tumours in the clinical application due to a high contrast between malignant and healthy tissue.
Afterwards, bacterial cells were incubated with 400 µl washing solution (10 mM Tris HCl, 0.9 M NaCl, pH 8.0) at 50 °C for 30 min to remove non-specific binding of the probes.
The excess probes are removed followed by binding of the probe-target complexes on the surface of the nCounter cartridge via a streptavidin-biotin linkage.
Some of these ALDHs, however, could not be confirmed as binders of the natural product ACV, suggesting that structural perturbations directed the binding of the probes to ACV other enzymes.
