Sentence examples for binding of the polyclonal from inspiring English sources
Thus, binding of the polyclonal antibody appears to inhibit conformational changes in the protease.
Mutations at I116H, I117P, P118S and W122G resulted in complete loss of AVFluIgG01 binding; moreover, mutations at S120N and S121A resulted in substantially reduced binding of AVFluIgG01, but had no impact on the binding of the polyclonal patient's serum (Table 6).
The polyclonal antibody is directed against many epitopes and it appears that some of these epitopes cause binding of the polyclonal antibody to regions of the BoNT/A1 molecule that also interfere with its ability to interact with its peptide substrate.
We confirmed that this internalization process is not an artifact triggered by the binding of the polyclonal antibody itself by performing internalization assays using the Fab fragments derived from the affinity-purified-specific antibodies (supplementary Fig. 2).
The N-domain specific monoclonal antibody (mAb) YTH71.3 has been shown to require Gln-89 and Ile-91 for receptor recognition and substitution of these amino acids had no effect on the binding of the polyclonal anti-CEACAM antibody A0115 (Virji et al., 1999).
The weak immunoreactivity to anti-gliadin antibodies showed by rice digests might be due to a nonspecific binding of the polyclonal antibodies (reacting not only to toxic sequences) to rice proteins, whereas the R5 antibody is a monoclonal antibody that recognizes very specifically the gluten toxic sequence QQPFP [ 35].
These revealed that a number of different regions on TNFα are involved in binding to the polyclonal Fab.
The specificity of the biosensor was based on the unique binding characteristics of the polyclonal and monoclonal antibodies immobilized on the immunosensor.
Competitive ELISA using antibodies from a chicken injected with α-peptide 1 conjugated to the carrier show that free peptide 1 disrupts binding of these polyclonal antibodies (anti- 1) to immobilized 1 more effectively than α/β-peptide 2 disrupts this binding.
The observation of multiple bands in pDis/H5 (66 kDa) might be due to nonspecific binding by the polyclonal antibody used in the studies.
The antigen mixtures are expected to have a variety of unique epitopes that would enhance binding of the broad spectrum of polyclonal anti-AMA1 antibodies in naturally exposed individuals.
