Sentence examples for binding of the phage from inspiring English sources
Furthermore, the result that the chemically synthesized APWHLSSQYSRT peptide could inhibit binding of the phage to ES cells indicated that the specific binding ability of APW phages was due to the displayed peptide and independent of the phage itself.
At 2.5 V, pixelated green fluorescent signal from individual wells was identified with some nonspecific binding of the phage to the PMMA between the nanowells.
Specific binding of the phage particles displaying scFv antibodies was measured by FACSArray or FACSCalibur instrument (BD Biosciences, Franklin Lakes, NJ).
To eliminate nonspecific binding of the phage to BSA, another plate coated with 100 μL of 3% BSA in PBS was used for preabsorption.
Since electrostatic binding of the phage to the target protein weakens with increasing ionic strength, which in turn influences the specificity of the interaction [ 39], different buffers with different ionic strengths were tested prior the actual panning experiments.
Tail fibers and tailspikes are appendages in the phage tail that facilitate the initial binding of the phage to the bacterial host; they hence represent primary receptor-binding proteins.
After the plates were washed five times with PBST, binding of the phages to the peptides was detected with a rabbit antiserum against the T7 phage (a kind gift from Dr. Toshiyuki Mori at National Cancer Institute, Frederick, MD) and a secondary antibody conjugated with horse radish peroxidase (HRP) (Sigma).
The low magnification fluorescent image shown in Figure 3b confirms that the trapping of the phages was confined to the PC structure region and that nonspecific binding of the phages onto the surface of the PMMA between the PC structures was absent.
His group was able to predict conformational changes of pRNA upon binding to the phage procapsid.
These data demonstrate that the binding ability of the phage to ES cells is caused by it displaying the APWHLSSQYSRT peptide.
The binding efficiency of the phage pool was calculated by the ratio of its output phage number to input phage number in each round selection, in order to survey the effect of selection.
