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We infer that binding of the ordered population reflects structural changes induced on the outer surface as pressure builds up inside the capsid during DNA packaging.
The Thr to Leu substitutions disrupt the binding of the ordered water to sbwAFP by removing the stabilizing hydroxyl side chain hydrogen bond interactions of the threonine residues.
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Under greater σhφ values, not only the binding rates of the disordered system is significantly affected, but also the binding rate of the ordered system is affected.
Arrays of positioned (or "phased') nucleosomes are found surrounding the nucleosome-free CTCF-binding sites [ 11- 13], suggesting that CTCF binding promotes the ordered positioning of histones in its vicinity.
It is important to note that even with the significant pairwise similarity in promoter binding there were pairwise differences in promoter binding on the order of 36 to 100% between all FFRPs.
Our only evidence of the ordered product release is the data showing that CoA-SH binds to AANATA in the absence of N-acetyltyramine and is consistent with the ordered binding of substrates, acetyl-CoA binding first.
Besides we note the presence of large exciton binding energies, of the order of 1.5 eV, similarly to other highly confined Si and Ge systems [32, 58 60].
It quenches the fluorescence of CTX-M-15 spontaneously with binding constant of the order of 103 M−103
Analysis of fluorescence quenching data of HSA at different temperatures using Stern Volmer methods revealed the formation of AHHPPC HSA complex with binding affinities of the order 104 M−104
However, these small molecules caused a less severe and variable decrease in TAg ssDNA binding regardless of the order of ATP addition.
Additional hydrogen bonding interactions of the protein with GDP may account for the ordered binding of substrates to the enzyme.
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