Sentence examples for binding of the negative from inspiring English sources

To quantify this data, the percentage of positive cells that specifically bind the fusion protein, relative to the nonspecific binding of the negative control (A192), was gated for each cell type.

In support of this idea, recently the sugar laminaribiose was identified as an inducer (by inhibiting binding of the negative regulator GlyR3) of the celC gene cluster encoding non-cellulosomal enzymes in C. thermocellum [39].

Despite reacting with these parasite lines by flow cytometry (Table 1), rabbit anti-DBL1-6 plasma or purified IgG did not significantly inhibit binding of heterologous CSA-binding lines to CSA compared to the binding of the negative control CD36 binding parasite line to the CD36 recombinant protein (Figure 4B,C).

It should be noted that, after subtraction of the background binding of the negative control protein (Trx), the binding affinity of the peptide P1 to the human antisera after vaccination increased only about one fold compared with the antisera before vaccination (Fig. 6, panel D).

In addition, binding of the negative mark H3K27me3 featured prominently over the first half of the gene in hTERT-HPNE cells (Additional file 10: Figure S4).

Whelan et al. indicated that in prostate cancer cells and 293 cells Notch1 promoted PTEN gene expression through binding of the CBF-1 transcription factor to the PTEN minimal promoter, while in T-ALL cells Palomero et al. described that PTEN expression was decreased by Notch1 signaling, which was mediated by binding of the negative regulator Hes-1 to the PTEN promoter.

Interestingly, Drosha binding coincides with the binding site of the negative elongation factor NELF, which defines the "late" elongation checkpoint, ensuring conversion to productive elongation mediated by phosphorylation of Pol II CTD, NELF, and DSIF by the positive transcription elongation factor b (P-TEFb) (Egloff et al., 2009).

The absorbance of each plasma sample was calculated as a fraction of the mean absorbance value of the positive controls after the background level of non-specific binding (absorbance of the negative controls) was subtracted from all samples.

Subsequently, specific antibody-binding capacity was obtained after corrections for background, apparent antibody-binding capacity of the negative control mAb anti-Thy-1.2.

The Rif1 and Rif2 proteins have been proposed to represent a 'counting mechanism' for telomere-length homeostasis in which decreased binding of the Rif proteins – negative regulators of telomerase – leads to increased telomerase recruitment at short telomeres (Marcand et al., 1997; Levy and Blackburn, 2004; Teixeira et al., 2004; Bianchi and Shore, 2007).

In addition, CTCF bound to the segment of the human apoB gene (lanes 6 and 7 in Fig. 4A) as the positive control [32] and a rabbit IgG binding as the negative control of gel-supershift analysis (Fig. S3) in EMSA.