Sentence examples for binding of the myosin from inspiring English sources

However, excess Unc45b was used in these assays to maximize binding of the myosin subfragments.

Thus, binding of the myosin motor domain by Unc45bFlag/Hsp90 complex is limited to non-native conformations of the motor domain, characteristic of a chaperone activity.

It is believed to stabilize the binding of the myosin head to the core of the thick filament.

The subsequent, critical step in the force-generating cycle is the binding of the myosin head to the actin filament, forming a so-called cross-bridge.

As detailed in the Supplemental Information, these experiments clearly showed that the V480R/V484R mutant hinders binding of the myosin substrate in addition to blocking chain formation.

Indeed, systematic UNC-45 pull-down experiments, which were carried out at different temperatures and using cell lysates from unc-45 m94 unc-45 m94type worms reveandd that the distinct mutations affect the binding of the myosin substrate to different degrees.

Upon binding of ATP, the myosin head detaches from an old binding site on actin, reattaches to a new actin monomer, and when ATP is hydrolyzed changes conformation causing sliding of actin with respect to myosin [1].

Moreover, biochemical data suggest that the She4 UCS domain accommodates a 27-residue epitope of a yeast myosin V protein, which is located in close proximity to the nucleotide-binding and actin-binding sites of the myosin motor domain [ 26 ].

For example, Rho kinase is a serine/threonine kinase that regulates contractile events at the actin cytoskeleton primarily by phosphorylating and thereby inactivating the myosin binding subunit of the myosin phosphatase complex (reviewed in [15]).

However, since HA-1077 and Y-27632 are not highly selective for ROCK-I and ROCK-II, when ROCK-I and ROCK-II were suppressed by HA-1077, downstreamoleculesesuchch as the myosin binding subunit of the myosin light chain (MLC) phosphatase (MYPT -1 and LIN-11, Isl1, and MYPT -1omand kinase (LIMK) might compensate for ROCK inhibition [ 38].

Furthermore, effective interhead cooperativity may also require binding of the two myosin heads to neighboring actin filaments at interfilament distances similar to that in muscle [ 160] or there may be other forms of interhead cooperativity where the presence of two heads facilitates attachment of one of them but where sequential force-producing actions are not occurring [ 168, 340, 341].