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Importantly, antigen binding of the modified Fc was not affected by the engineered disulfide bonds.
By contrast, drugs lacking a formal positive charge, including CCBs (felodipine, nifedipine, diltiazem, verapamil) and an angiotensin-converting enzyme-inhibitor (ramiprilate) had no effect on the column binding of the modified, electronegative lipids.
Substitution of the P-site tRNA A76 2′ OH with 2′ H or 2′ F results in at least a 106-fold reduction in the rate of peptide bond formation, but does not affect binding of the modified substrates.
These effects were due to high binding of the modified albumins to the injured vessel.
Peptide P27 has the highest affinity coupled with highest specificity for binding of the modified hASLLys3UUU.
Equilibrium binding constants (as the dissociation constant Kd; Table 4) were determined from the concentration-dependent fluorescence quenching with the binding of the modified and unmodified hASLLys3UUU.
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They argued that this asymmetry arises because many mutational routes to receptor modification or loss can achieve resistance, while phage infectivity requires the evolution of specific binding to the modified version of the existing receptor or to an entirely different receptor, which is likely to be subject to greater mutational constraint.
We analyzed the ligand binding properties of the modified avidins with a fluorescent biotin conjugate, BF560-biotin, by measuring the quenching of fluorescence after protein binding at RT.
However, the binding affinity of the modified EGF to hydroxyapatite and titanium was significantly higher than the unmodified EGF.
Because of the extremely high binding capacities of the modified surfaces, the achieved yield of nucleic acids is practically unlimited, unlike that obtained through magnetic particle-based extraction, for example.
However, this modification severely reduced the binding affinity of the modified biotin for avidin.
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