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Molecular modeling studies were carried out to ascertain the binding of the model compound to the active site of β-tubulin.
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The VTD binding residues of the model peptide were identified using the NMR titration experiments with VTD, including a newly discovered VTD binding residue Leu14 (μ1-L1580 in Nav1.4), which has not been reported by point mutation studies.
Comparative modelling of the 3-D structure of LDGLOI shows that substrate-binding region of the model involves important differences compared to the homologues, such as E. coli, specific to glutathione.
In the absence of helix assembly, F1 is connected to the membrane through a small subset of positively charged residues at the end of the highly dynamic F1 loop, corresponding to the intermediate-binding stage of the model.
To extrapolate high-dose antibody residence at the tumor from low-dose estimates of binding parameters, the model must be able to account for decreased concentration of available antigen as a function of dose [22].
The simulation also showed that the binding free energies of the model and the known complex were in close agreement.
The technique is based on a multiparametric analysis of the nuclear binding of a model chemotherapeutic agent, the fluorescent dye Hoechst 33342 (Ho342), which is capable of supra-vital staining of DNA in intact, viable cells.
The relative strength of binding of each model was assessed with the "fastcontacts" server [34].
An alternative organization of the full-length dimer would involve binding of the bridge pair in Model 1 to the other (DID-DD 2 DID-DD 2r equivalently bindimeror thequivalentlydimer in Model 1 to the other bindingpair) (Fig. 3C, Mofel 2).
In Figure 3(C), the binding state of the initial model is compared with that of the MD-simulated model at 15000 ps.
We also investigated the functional capacity of CS-A-bound C1q with regard to the binding of model immune complexes to the platelet surface.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com