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In the present study, the pentameric chlorite dismutase (Cld) from the bacterium Candidatus Nitrospira defluvii was probed for binding of the low spin ligand cyanide, the substrate chlorite, and the intermediate hypochlorite.
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In any case, Table 2 demonstrates that the impact of exchange of arginine 173 on the catalytic efficiency of chlorite degradation is significantly smaller than on the binding of the low-spin ligand cyanide.
Next, we probed the effect of binding of the low-spin ligand cyanide on the redox properties of the Fe(III)/Fe(II) couple, performing redox titrations of cyanide complexes of both enzymes at different applied potentials.
In this work, we study the binding of the low-spin ligand cyanide, a known inhibitor for heme oxidases, the substrate chlorite and the postulated intermediate hypochlorite to wild-type NdCld and the mutant R173A by means of molecular dynamics simulations in order to gain more information about the role of the flexible Arg173.
In the case of tra F, this might reflect a low-level of U2AF binding to the low affinity site, even in the absence of Sxl.
The lower hinge region of the antibody contributes most of the binding to the low affinity Fcγ receptors.
These methods are particularly effective at detecting the binding of low affinity, low molecular weight compounds and transforming them into novel potent leads using structure-guided chemistry.
The absence of effect on the binding to the low affinity site from the grafted fluorescein suggested that this site was distant on the DNA from the high affinity site [43].
In marked contrast, the binding of this low concentration of CA200645 (5 nM) was insensitive to inhibition by the agonist NECA at concentrations of up to 300 nM.
By subtracting the binding energy of the low energy cutoff from the high binding energy edge of the UPS spectra, the work function of the sample is obtained [8].
FGL2 inhibits dendritic cell maturation and induces apoptosis of B cells through binding to the low-affinity FcgammaRIIB receptor, and thus contributes to Treg cell activity [33].
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