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In all systems studied to date, binding of the light chains to the heavy chain is so tight that dissociation constants have not been measured.
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Here, we describe the mechanism of light-dependent DNA binding of the light-oxygen-voltage (LOV) transcription factor Aureochrome 1a from Phaeodactylum tricornutum (PtAu1a) and present crystal structures of a dark state LOV monomer and a fully light-adapted LOV dimer.
In tumor cells, cathepsin B redistributes into exocytic vesicles at the cell periphery leading to its secretion and association with the tumor cell surface by binding to the light chain of the annexin II heterotetramer [ 8, 9].
We then investigated the consequences of this tBid binding for the light-scattering properties of CL-GUVs.
Genes encoding the chlorophyll a/b-binding polypeptides of the light harvesting system (Lhca/b, also called cab) are controlled by the circadian clock in some plant species [ 58- 61].
Changes in amino acids surrounding the binding pocket of the light-sensitive chromophore of the opsin can directly alter the spectral sensitivity of the visual pigment.
According to the integrative database of germ-line variable genes from the immunoglobulin loci of human (VBASE2) the isolated scFv fragments contained the antigen-binding variable domains of the light chains LV1, 2, 3 and 6.
Molecular modeling results suggested that framework region (FR) residues latently important to antigen binding included residue 49 of the light chain variable region (VL).
The transcriptional phase of these target genes is heterogeneous and distributed throughout the day, despite a more discrete phase of BMAL1 DNA binding at the beginning of the light phase.
However, the competiton ELISA data demonstrated that R145 and S146 do contribute in part to the binding of F1-40 thethe light chain of BoNT/A, under the solution conditions of competition ELISA.
Mutation of Q139, P140 and D141 to glycine residues or eliminating the entire loop (mutant LcΔ) abolished binding of F1-40 thethe Lc peptide in both the competition ELISA and Western blotting experiments, indicating that the QPD triad is necessary for the binding of F1-40 thethe light chain of BoNT/A.
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