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This pathway is activated by binding of the ligands including sonic hedgehog (Shh) to its membrane receptor patched 1 (Ptch1).
Mutants with reduced kinetic capabilities were analyzed further to study the effect of the amino acid alteration on the binding of the ligands.
For example, binding of the ligands could trigger the conformational changes in some of the Trypanosomal enzymes to modulate their activity leading to the disease pathogenesis [48], [49].
If α < 1, there is negative cooperativity in binding of the ligands.
The binding of the ligands stimulates receptor dimerization, the critical step for activation of tyrosine kinase activity.
The cells were then analyzed by flow cytometry to detect PE signals from binding of the ligands on cells.
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The relationship between ligand and transportate properties indicated that the initial binding of the ligand to hPEPT1 is the major determinant for translocation of the investigated dipeptides.
Do these really pinpoint the actual functional groups involved in the non-covalent binding of the ligand to the active site?
Although the global motion of SH2 domain regulates Csk function, little is known about the relationship between the disulfide bond and binding of the ligand.
Physiologically, GABA receptors are activated upon binding of the ligand, GABA.
The binding of the ligand results in the dissociation of the trimeric G-protein.
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Justyna Jupowicz-Kozak
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