Sentence examples for binding of the labeled from inspiring English sources

Therefore, the excess quantity of the non-labeled probe interfered with the specific binding of the labeled MYC4 promoter fragment.

The unlabeled antibody was considered to bind to the same epitope as the labeled antibody if it reduced binding of the labeled antibody by at least 75%.

SPEFS was used to follow the kinetics of the binding of the labeled fatty acid to the antibody, and to implement a competition assay with free fatty acid (undecanoic acid), sensitive at the 1 μM scale, a sensitivity limit caused by the low affinity of antibodies for free fatty acids, rather than the SPEFS technique itself.

Solid phase radioimmunoassay was used to evaluate the in vitro binding of the labeled antibodies to a human breast tumor metastasis to the liver, the MCF-7 breast tumor cell line and normal liver tissue.

For example, ex vivo assays are optimized so maximum binding of the labeled probes can be obtained in the excised sections and are not performed under the same conditions that affect probe accessibility in vivo.

The mean fluorescence intensity of the unselected library was subtracted from that of the corresponding aptamer with the target cells to determine the specific binding of the labeled aptamer.

In all cases each EMSA test was repeated more than two times to ascertain the reproducibility of the overall pattern of nuclear protein binding to the labeled oligonucleotides.

Native gel shift assays using 4 12% Tris-glycine gels (Invitrogen) were performed to assess binding activity of the labeled proteins.

The true IGF-I concentrations are approximately twofold higher than the results obtained using the immunoassay, most likely because of a lower binding affinity of the labeled antibody in this human IGF-I immunoassay.

Mnv agents EUKL-DOTA 3, cRGDfK-DOTA 5, and cRGDfK-IRDye800 6 were synthesized as outlined in Schemes 1 and 2. They represent the controls to which the in vitro binding affinities of the labeled HtBv agents could be compared.

The fluorescence signals emanating from the antibody complexes that were used to monitor phosphorylation of the three tyrosine residues cannot be compared because of the different affinities of the primary antibodies for the epitopes, and distinct fluorescence brightness and binding affinity of the labeled secondary antibodies.