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For example, deletions of alb1 and arp1 significantly increase the binding of the human complement component C3 to A. fumigatus conidia [46], [51].
To gain further insight into the molecular basis of complex formation between these two proteins we measured binding of the human and mouse NOXA1-SH3 domains to either NOXO1-SH3AB E construct by ITC.
Moreover, the binding of the human scFvH5 with yCD did not affect the enzyme function.
10.7554/eLife.07702.007 Figure 2. Rb1 hyperphosphorylation and E2f1 binding of the human ARF promoter in the blastema during regeneration.
It has been reported that binding of the human MC1R by its ligands can activate the cAMP signaling pathway and regulate pigmentation of human melanocytes [ 31].
DOI: http://dx.doi.org/10.7554/eLife.09811.009 We next examined binding of the human ALK ECD with purified FAM150A by ELISA and Biacore surface plasmon resonance (SPR) analysis.
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This demonstrates that individual features of the native and hydroxylated estrogens determine the specific binding properties of the compound within the binding site of the human UGT1A10 and the mutants.
Initial experiments were performed to characterize the binding parameters of the human α7-nAChR heterologously expressed in the rat GH4-hα7 cell line using a live-cell binding method.
We have designed a combinatorial library based on the DNA binding domain of the human retinoid-X-receptor (hRXRα).
The binding abilities of the human HAs displayed by yeast cells were confirmed by the hemagglutination assay described in "Materials and methods".
Alignment study of docked quinazoline 3 into the active binding pocket of the human cyclin-dependent kinase 2 enzyme (Fig. 5c) revealed arene-hydrogen interaction with bond length of 4.23 Å and binding energy of −0.6 (kcal/mol) with Ile10.
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