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Exact(35)
The bacterial DNA inhibited the binding of GST-ComE1 to Fn indicating that not only does GST-ComE1 bind to DNA, but DNA appears to be a better inhibitor of the binding of the fusion protein to immobilised Fn than soluble Fn (Fig. 1a).
MLL-Af4 induces a B ALL distinct from MLL-AF9 through differential genomic target binding of the fusion proteins leading to specific gene expression patterns.
Upon binding of the fusion protein to the cell of interest, the diphtheria toxin is internalized which results in protein synthesis inhibition and subsequent cell death.
The binding of the fusion construct to the angiotensin receptor (AT1) was similar to AngII, and the arterial contraction was 16 ± 1% of the response observed with norepinephrine.
The modified intein contained a Bacillus circulans chitin-binding domain which allowed binding of the fusion protein on a chitin column and could be induced to undergo in vitro peptide bond cleavage which specifically released Cre from the column.
The no-Ter/wild-type Ter signal ratio (−TER/+TER) is 0 for Cy5 and 0.12 for Cy3, consistent with significantly higher binding of the fusion protein to plasmid containing wild-type Ter as compared to plasmid without any Ter.
Similar(25)
The four antigen-binding and biotin-binding sites of the fusion protein retain the functional capabilities of the parent molecules (Schultz et al, 2000).
Although, the binding specificity of the fusion proteins is not impaired, a higher number of tryptophan residues result in a decrease of the binding activity.
The mismatch binding properties of the fusion protein were confirmed by DNA mobility shift assay in polyacrylamide gels.
In addition, the structural difference of fusion tags affects the binding strength of the fusion protein, which can influence the resulting product purity.
The binding characteristics of the fusion construct were unaltered by the addition of the AngII sequence [affinity constant KA 1.18 × 107 and 8.42 × 106 M−1 for sc(Fv 2 and sc(Fv 2-AngII, respectively].
More suggestions(16)
binding of the anti-fibrinogen
binding of the outbreak
binding of the buffer
binding of the myosin
binding of the p57T143A
binding of the peptide
binding of the radiolabel
binding of the heterodimer
binding of the target
binding of the ligand
binding of the hormone
binding of the karma
binding of the action
binding of the initiator
binding of the nation
binding of the rabbit
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