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The retention of the constituent fragments was then compared to that of the mAb to gain insight into the relative importance of these different domains and the contribution of each domain to the binding of the full mAb in these systems.
Since the approach of binding studies of the isolated, epitope-labelled domains to suspended cells is very artificial we further characterized and compared binding of the full length and truncated TcdA to cell surfaces of intact cells.
As shown in Fig. 5D, the truncated domain of FKBP52 bound APP in a similar fashion to the binding of the full length protein, suggesting that the interaction of FKBP52 with APP is mediated by the PPIase domain.
Interestingly, as confirmed by both approaches, CHO cells showed enhanced binding of truncated TcdA and no binding of the full length protein (Fig. 5B, bottom panel and Fig. 5C, lower panel) though being identical susceptible towards both toxins (compare Fig. 3).
PTB binding of the full(A) mutant RNAs was identical to that observed for the synonymous mutations, showing that the residual binding observed for the synonymous mutant RNAs was not due to remaining pyrimidines in the potential binding sites (data not shown).
To our knowledge, the direct binding of the full VT to Tie2 has not been formally described or quantified.
Similar(53)
Furthermore, both of the IN60 80 and IN231 251 peptides significantly interfered with the binding of the full-length IN (His-WT) to the SIP1 protein.
The first step of intoxication involves binding of the full-length, 83 kDa form of PA (PA83) to either of two cell surface receptors, ANTXR1 (anthrax toxin receptor/tumor endothelial marker 8; ATR/TEM8) or ANTXR2 (capillary morphogenesis gene 2; CMG2) [4], [4].
ITC demonstrated binding of the full-length hEdc3 to NADH but not to the other tested metabolites.
Although there is higher affinity binding of the full-length protein when PtdIns 3,4,5) P3 is present, there is no change to Δ f.
Reciprocal caspase-3 pull-down (IP-Cas-3) confirmed the highly specific binding of the full-length and partially processed caspase-3 to this complex in only WT cells.
More suggestions(14)
binding of the active
binding of the fourth
binding of the targeted
binding of the immunoconjugate
binding of the secondary
binding of the polyclonal
binding of the hybrid
binding of the former
binding of the different
binding of the non
binding of the same
binding of the mutant
binding of the agonist
binding of the entire
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