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The binding of the enzymes was compared after a certain time of incubation (i.e. at a single time point) as binding kinetics was not the focus of this study.
The use of MQ-water, instead of buffer, is deemed justified as in this work we are interested in the binding of the enzymes, not their activity which can be strongly affected by pH.
The flexibility of IDRs facilitates the binding of the enzymes that bring about the disorder-associated posttranslational modifications.
The surfactant may also display a competitive adsorption with the cellulolytic enzymes, thus reducing the binding of the enzymes on the lignin [ 28].
The LysM domains of bacterial cell wall hydrolases help in binding of the enzymes to their substrate peptidoglycan and thereby facilitate cell wall hydrolysis.
The increase in glucose production depends on availability of cellulose in the medium and also due to the specific binding of the enzymes with substrates [ 34].
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Other experiments revealed that the addition of heparin inhibits the binding of the enzyme to endothelial cells suggesting that XDH/XOD binds to the polysaccharide chains of heparin-like proteoglycans on the endothelial-cell plasma membrane [ 10].
The biosensor was constructed by covalent binding of the enzyme substrate d-fructose 6-phosphate (F6P).
Considering the possible non-specific binding of the enzyme and proteins in blood, which may influence the specific analysis, we used BSA to check this effect.
Exocellulase enzyme binds to multiple cellulase chains (three chains in the model) (Asztalos et al. 2012; Levine et al. 2010), so it is essential that all three chains must be accessible to the enzyme (on surface and not blocked by other enzyme) for binding of the enzyme.
The binding of the enzyme to the Con A-agarose column indicated that the peroxidase was a glycoprotein compatible with the previous studies reporting that most of the plant peroxidases were glycoproteins (Johansson et al. 1992; Kvaratskhelia et al. 1997; Passardi et al. 2005).
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CEO of Professional Science Editing for Scientists @ prosciediting.com