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Other experiments revealed that the addition of heparin inhibits the binding of the enzyme to endothelial cells suggesting that XDH/XOD binds to the polysaccharide chains of heparin-like proteoglycans on the endothelial-cell plasma membrane [ 10].
The biosensor was constructed by covalent binding of the enzyme substrate d-fructose 6-phosphate (F6P).
Considering the possible non-specific binding of the enzyme and proteins in blood, which may influence the specific analysis, we used BSA to check this effect.
On the other hand, immobilized peroxidase showed a slightly lower inactivation rate, suggesting no significant protection of the enzyme against hydrogen peroxide, due to the binding of the enzyme to PS matrix as shown in Figure 7. Figure 7 First-order rate constant calculations from semi-logarithmic plot of residual activity of soluble and immobilized peroxidase with H 2 O 2 incubation.
The C-terminal end of Clr4 harbors a SET domain responsible for the enzyme's catalytic activity, while the N-terminal domain contains a chromodomain region that mediates binding of the enzyme to H3K9 methylation groups (Min et al., 2002; Zhang et al., 2008).
The binding of the enzyme to the Con A-agarose column indicated that the peroxidase was a glycoprotein compatible with the previous studies reporting that most of the plant peroxidases were glycoproteins (Johansson et al. 1992; Kvaratskhelia et al. 1997; Passardi et al. 2005).
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The binding of the enzymes was compared after a certain time of incubation (i.e. at a single time point) as binding kinetics was not the focus of this study.
The use of MQ-water, instead of buffer, is deemed justified as in this work we are interested in the binding of the enzymes, not their activity which can be strongly affected by pH.
The flexibility of IDRs facilitates the binding of the enzymes that bring about the disorder-associated posttranslational modifications.
The surfactant may also display a competitive adsorption with the cellulolytic enzymes, thus reducing the binding of the enzymes on the lignin [ 28].
The increase in glucose production depends on availability of cellulose in the medium and also due to the specific binding of the enzymes with substrates [ 34].
More suggestions(18)
bonding of the enzyme
binding of the inhibitor
binding of the protein
binding of the inhibitory
binding of the antibody
binding of the viral
binding of the anti-fibrinogen
binding of the p57T143A
binding of the peptide
binding of the radiolabel
binding of the heterodimer
binding of the action
binding of the karma
binding of the initiator
binding of the rabbit
binding of the outbreak
binding of the buffer
binding of the myosin
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