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Target specificity of the lead compound was established by demonstrating direct binding of the compound to Magmas and by genetic studies.
The different effect of reagents able to link to one cysteine - such as MTSET, MTSPT, MTSPtrEA - and those able to link to two cysteines - M-X-M cross-linkers - with the same size and volume was used to discriminate whether blockage was caused by bridging two cysteines by the binding of the compound to one end only.
The molecular docking studies of compound 9j indicated the binding of the compound at the active site of recombinant human ROCK II which makes it a promising antiglaucoma agent.
Introduction of the I194F substitution in the model of VP1 induced a slight concerted rearrangement of the core β-barrel in this pocket, which disfavors binding of the compound.
Mutations conferring resistance to DSB also led to impaired binding of the compound to immature HIV-1 virions and loss of DSB-mediated inhibition of cleavage of Gag.
The binding of the compound is believed to be occurring with the large nuclei of the cells.
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To explore the relative binding of the compounds in living cells, we performed FRET studies in U2OS cells.
This assay was used to measure the binding of the compounds to sGCcat.
The non-specific binding of the compounds to the plastic surfaces in DC and RC was determined to improve the accuracy of the subsequent quantitative analyses (Table 1).
The bioaffinity traces with increasing peak maxima correspond to the dose-dependent increases in binding of the compounds to the receptor.
We first compared the rapid binding of antimycobacterial compounds to activated charcoal or bovine serum albumin fraction V (BSA) and then tested the impact of incubation time on the binding of the compounds to activated charcoal.
More suggestions(17)
binding of the antibody
binding of the viral
binding of the anti-fibrinogen
binding of the p57T143A
binding of the peptide
binding of the radiolabel
binding of the heterodimer
binding of the action
binding of the karma
binding of the initiator
binding of the rabbit
binding of the outbreak
binding of the buffer
binding of the myosin
binding of the fusion
binding of the target
binding of the ligand
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