Here, we relate an atomic-detail model that has been developed to describe the transcription factor NikR's mechanism of DNA binding to the broader theories of how transcription factors find their binding sites on DNA.
These common features observed in X-ray structures predict that allosteric functional linkages between tRNA and amino acid binding, and the broad dispersal of amino acid specificity determinants, will also be prevalent if not universal among the aaRS.
The conformational adaptability and the allosteric fatty acids-induced regulation facilitate the binding of a broad range of pharmaceutical drugs, affecting their availability and biological efficacy (Curry 2009).
G-protein-coupled receptor binding (purple, Figure 3a), part of the broad functional category of signal transduction, differed between CFS subjects and controls prior to exercise.
Here, we analyse the binding of the P14 soluble TCR to a broad panel of related H-2Db-peptide complexes by surface plasmon resonance, and compare this with their diverse cellular responses.
For example, positive selection was detected at amino acids involved in ligand binding of the frog PXRs, which have lost broad specificity for ligands and gained efficient activation by endogenous amphibian-specific benzoates [ 55].
A range of binding profiles is observed, from very restricted binding to a small set of structurally similar ligands to binding of broad classes of ligands with related terminal sugars and even to failure to bind any of the glycans on an array.
The RGG box is typically associated with binding to single-stranded nucleic acids, (Zhang and Grosse, 1997) whereas additional RNA-binding motifs may allow binding of a broader range of RNA targets as is the case for nucleolin (#33, Table S1) (Ghisolfi et al. 1992).
This binding is explained by the broad binding affinity of lectin.
All aptamers were characterized under identical conditions (10 mM HEPES, 150 mM NaCl, and 5 mM MgCl2 at pH 7.4 and 25 °C), enabling the first direct comparison of the binding properties of a broad set of small molecule-binding aptamers.
