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Stronger binding of the analogs was also inferred from circular dichroism studies and thermal melting experiments.
We find differential binding of the analogs to the receptors after photobleaching and a dependence of the binding kinetics on the oligomerization state of the protein.
Binding of the analogs to the adjacent sites of a target biopolymer brings the pre-reactive groups in close proximity and causes their interaction followed by covalent damage of the target.
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The binding of these analogs to poly(U) and poly(C) was weaker in comparison to poly(G) and poly(I) but were one order higher in comparison to berberine.
Our results show that the binding of the HRC analogs to HRN does not correlate with the coiled-coil stability of the HRC analogs, but their interactions with HRC does correlate with their stability, except for HRC7.
This supports the key role of Arg195 in 3β-HSD1 for the high affinity, competitive binding of the trilostane analogs.
These observations support the hypothesis that moderation of the D2 binding of the tropane analogs could reduce catalepsy potential in rats and consequently EPS in man.
Furthermore, AutoDock study has been done by binding of the flavin analogs into PTK pp60c-src, where a good correlation between their IC50 and AutoDock binding free energy was exhibited.
To compare the binding of the ATP analogs with ADP, we performed competition experiments in which the ATP hydrolysis activity of RIG-I was evaluated in the presence of ATP and each of the nucleotide analogs that were used in this study (new data shown as Figure 5 figure supplement 1).
UV melting experiments indicate the formation of a well-defined triplex with specific binding of the urocanamide analog to a CG inversion of the homopurine homopyrimidine target.
Both peptides 14a and 14b provided DNA retardation indicating binding of the IHF analog.
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