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The time-resolved fluorescence decay analysis was used to investigate the specificity and affinity of the binding of template molecules to the MIP.
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It is obvious that the binding amount of template molecules to MIP nanoparticles were much higher than that of NIPs.
After extracting the embedded template molecules, the produced imprinted Fe3O4@polynorepinephrine (MIP–Fe3O4@PNPs NPs have cavities complementary to three dimensional shape of template molecules favoring high binding capacity and magnetism property for easy manipulation.
This indicates that, at higher concentrations, the presence of a large number of high-affinity binding sites on the surface of Myr-NQA-MINs offers more opportunity for rapid adsorption of template molecules.
Thus number of template molecules didn't influence significantly mutability level.
This approach is of interest since the production of template molecules is expensive and laborious.
Pharmacophore was generated by collecting a common set of template molecules structural features.
There was no significant loss of template molecules with these two genes during the assay process.
Non-imprinted polymers were prepared following the same procedure but in the absence of template molecule.
The obtained imprinting material by using the new surface molecular imprinting techniques possesses superexcellent binding property for template molecules or ions because of the distribution of imprinted cavities in a thin polymer layer and smaller diffusion barrier.
The resulting MMIP showed high adsorption capacity, proper selectivity and fast kinetic binding for the template molecule.
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