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Recognition and binding of specific sites on DNA by proteins is central for many cellular functions such as transcription, replication, and recombination.
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It is now generally accepted that many of the physiological effects of alcohol consumption are a direct result of binding to specific sites in neuronal proteins such as ion channels or other components of neuronal signaling cascades.
NF-κB directly induces expression of A1/Bf1-1 bindinging to specific sites in its promoter [ 34].
A third complex consists of a DNA binding protein Pleiohomeotic (PHO) that interacts with PC and directs its binding to the specific sites of recruitment [ 6].
In agreement with experiments we observed that binding of specific peptides at this remote site leads to stabilization of the activation loop located in the active site.
This is where a DNA-binding protein is immunoprecipitated with its cognate DNA and the presence of DNA binding at a specific site is assessed by quantitative PCR.
The regulation of a gene depends on the binding of transcription factors to specific sites located in the regulatory region of the gene.
Consequently our simulation can reflect only on the initial events associated with the binding of Ca2+ to the specific sites.
We then used this luminescence signal to measure the binding affinity of Tb3+ to specific sites on SbpA.
For genes with higher expression levels the relative proportion of binding at non-specific sites is lower.
Therefore, expression regulation involves not only the binding of site-specific transcription factors/cofactors but extensive coupling and coordinating among relevant machineries and processes.
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