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Other regulation could include binding of specific sRNAs near the RBS thus preventing translation [ 42].
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However, these studies focused mainly on changes in expression of specific sRNAs, while research on the sRNA biogenesis and action pathways is very limited.
Precise post-transcriptional processing and high accumulation of specific sRNA sequences were observed.
These effector candidates will be among the first genes tested for functional analysis of specific sRNA-target pairs.
Nucleotide preference in the 5' position is the determining factor in plants for binding of sRNAs to specific AGO proteins [ 35].
The proportions of common and specific sRNAs in both the libraries were further analyzed.
We conclude that tobamovirus infection induces the stabilization of a specific sRNA pool by yet unknown effector complexes.
With this approach we were able to detect up to 15 ng of the specific sRNA.
The activity of CsrA is regulated by specific sRNAs (CsrB/CsrC).
Other stress conditions, such as anaerobiosis (CO2 atmosphere) and exposure to ethanol or peroxide (H2O2), downregulated specific sRNAs, but did not modulate expression profiles of other sRNAs.
Thus, although strong expression correlations, either positive or negative, were observed between specific sRNAs and their mother gene, the expression levels of most sRNAs were not correlated with their mother genes, suggesting independent transcription of sRNAs and mRNAs.
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