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The binding of samples (500 μM) to coated and uncoated reference sensors was measured over 120 seconds.
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The results concerning the induction of estrogenic biomarkers are in accordance with our findings for estrogen receptor binding of sample extracts from the Würm and sewage taken in parallel at the end of the experiment, when sewage extracts possessed a much higher ability to displace [3H]estradiol from the estrogen receptor than the ones extracted from the mixtures.
Teflon construction is used to minimize nonspecific binding of test samples to the apparatus.
An enzyme linked lectin binding assay (ELLBA) was performed according to Flesch et al. [19] to quantify lectin binding of the samples.
We describe here a proteome-wide approach to identify RNA-protein interactions based on in vitro binding of RNA samples to yeast protein microarrays that represent over 80% of the yeast proteome.
The clinical association and titre of anti-DI binding of serum samples in this study are shown in Table S1.
Blank wells coated with carbonate buffer without antigen were also prepared to test non-specific binding of the samples.
This may be due to unequal distribution of hybridization solution, spatial bias [ 17], non-specific binding of labeled samples to the array surface, or non-hybridized DNA not washed away [ 18].
Increasing the antigen amount from 50 ng to 100 ng resulted in enhanced detection of sera, especially for those showing moderate binding at the lower amounts, as exemplified by WNV serum 7 (see Figure 2), but increased the background binding of some samples (data not shown).
For example, Once we identify "Only" binding of genes in Sample A and Sample B, we are able to perform GO or other pathway analyses on these genes and may identify some interesting biological functions.
The binding of human serum samples to M2e-coated plates was compared to a standard curve of human polyclonal IgG.
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