[14] In the following examples, we prove that label-free MST is a valuable tool to study the binding of numerous types of ligands to different protein classes.
Very low density lipoprotein receptor (VLDLR) has been considered as a multiple function receptor due to binding numerous ligands, causing endocytosis and regulating cellular signaling.
The glycocalyx also modulates the inflammatory response by preventing leukocyte adhesion and binding numerous ligands, including chemokines, cytokines and growth factors [ 4- 6].
All members of the LDL receptor gene family harbor at least one structurally highly similar extracellular ligand binding domain consisting of a series of negatively charged cysteine-rich Ca2+-chelating repeat modules that bind numerous ligands [14].
Numerous pathways that promote cell proliferation are activated by the binding of various ligands to cell surface receptor tyrosine kinases (RTKs).
During evolution, this protein diversified into numerous families with low sequence similarity but with a common fold that succeeded as a versatile scaffold for binding of bulky ligands.
How binding of multiple ligands to receptors shapes dose responses.
For ALR2 and FGFR1, conformational changes in the binding site upon binding of different ligands were observed [30, 31].
Numerous ligands interact with the intracellular domain of the GluN2A subunits of NMDAR [ 32].
For numerous ligands, we can cluster them by the similarities of their 2D structures.
