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The best characterized example of this is the response to the binding of ferric citrate by FecA in E. coli.
An iron chelator nitrilotriacetic acid (NTA) [ 45] was employed to prevent unspecific binding of ferric ions to protein molecules.
Interestingly, increasing FeCl3 increases the ionic interaction at higher concentrations as these ionic interactions may disturb the conformation and favor the binding of ferric ions with the protein thereby decreasing ovotransferrin yield.
Binding of ferric citrate triggers a conformational change of the extracellular loops that close the external pocket of FecA.
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In this in vitro solution study, we quantified the binding of unchelated ferric iron to FbpABp in the presence of various anions and importantly, we demonstrated that FbpABp binds all the ferric siderophores tested (native and xeno) with μM affinity.
Methods for assessing the interferences by hydrogen ion, competing metal ions, hydrolysis, and precipitation are explained, and are applied to systems containing iron III) chelates of fourteen chelating agents designed for effective binding of the ferric ion.
The iron storage mechanism involves binding of ferrous iron to ferritin protein followed by migration to the ferroxidase catalytic site where ferrous iron [Fe(II)] is oxidized to the ferric [Fe III)] state.
The temperature was 37 °C in the case of the measurements involving substrate and product binding and reduction of ferric P450 4A11.
Consequently, we have limited ourselves to NO binding to ferric heme in this work.
For studies on cyanide binding to ferric wild-type NdCld and the mutants, the conventional stopped-flow mode was used, and the decrease of the absorbance at the respective Soret maximum was monitored.
It includes binding of chlorite to ferric Cld followed by subsequent heterolytic OCl bond cleavage leading to the formation of Compound I and hypochlorite, which finally recombine for production of chloride and O2.
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