Your English writing platform
Discover LudwigSuggestions(1)
Exact(2)
In order to visualize binding of each scFv, fluorescent images were taken.
Binding of each scFv is clear when pre-incubated with a secondary fluorescent antibody and then with target cells and imaged for fluorescent staining.
Similar(58)
To demonstrate the specificity of the Ab3 response against anti-Id scFv, an inhibition ELISA was carried out by inhibiting the binding of each anti-Id scFv on trastuzumab F ab′ 2 fragments by serial dilutions of sera from immunised mice.
First we analyzed the binding capacity of each purified scFv to the E7 protein covalently coupled to a CM5 sensor chip.
After binding of the scFv to Aβ monomers, the formation of aggregation seeds and potentially toxic higher order species, such as oligomers and fibrils, was inhibited [47].
The specific binding of the scFv antibody to the VNTR region of tumour-associated MUC1 was confirmed by ELISA (data not shown) and immunoblot (Fig. 2).
The scFv binds to an epitope on VEGF-C that is important for receptor binding, since binding of the scFv to VEGF-C dose-dependently inhibits the binding of VEGF-C to VEGF-R2 and VEGF-R3 as shown by BIAcore and ELISA analyses.
Specific binding of recombinant scFv was proven by flow cytometry.
The flow-cytometric analysis confirmed the specific binding of the scFv to the mTF antigen (Fig. 3a,b).
Inhibition ELISA was carried out by binding of chimeric scFv to SAI/II and replacement with an excess of mAb Guy13.
To characterise the anti-Id scFv, competitive ELISA experiments were carried out using either HER-2/neu ECD-Fc fusion protein to inhibit the binding of soluble scFv to trastuzumab F ab′ 2 fragments (Ab1) or purified scFv to block the binding of HER-2/neu ECD-Fc fusion protein to Ab1.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com