Sentence examples for binding of each phage from inspiring English sources

Single-residue alanine scanning mutagenesis of the CGRRAGGSC phage were produced and binding of each phage to immobilized IL-11Rα was tested in a functional assay (Table 2).

Therefore, lysate preblocking and phage preincubation was found to reduce the non-specific binding of background phage more effectively.

Furthermore, the result that the chemically synthesized APWHLSSQYSRT peptide could inhibit binding of the phage to ES cells indicated that the specific binding ability of APW phages was due to the displayed peptide and independent of the phage itself.

At 1 µM Dec-RVKR-cmk phage RMS-P3/RR binding was only half-maximal, whereas the unspecific binding of control phage RMS-P3/AA remained unaffected.

We used recombinant LRP6 E1-E2-Fc and E3-E4-Fc proteinselectelect from human synthetic antibody phage libraries and confirmed binding of isolated phage clones to LRP6 by ELISA and FACS [20].

Specific binding of the phage particles displaying scFv antibodies was measured by FACSArray or FACSCalibur instrument (BD Biosciences, Franklin Lakes, NJ).

To eliminate nonspecific binding of the phage to BSA, another plate coated with 100 μL of 3% BSA in PBS was used for preabsorption.

At 2.5 V, pixelated green fluorescent signal from individual wells was identified with some nonspecific binding of the phage to the PMMA between the nanowells.

The binding of M13 phage to amorphous and crystalline nickel boride nanoparticles was characterised with a Zeiss Axiovert 200 inverted fluorescence microscope equipped with a Zeiss AxioCam HRm.

Tail fibers and tailspikes are appendages in the phage tail that facilitate the initial binding of the phage to the bacterial host; they hence represent primary receptor-binding proteins.

Since electrostatic binding of the phage to the target protein weakens with increasing ionic strength, which in turn influences the specificity of the interaction [ 39], different buffers with different ionic strengths were tested prior the actual panning experiments.