Suggestions(1)
Exact(4)
By linking together two linear peptides to create a synbody, the probability that a synbody would bind a protein, PSyn, would be less than or equal to the product of the probabilities of binding of each peptide, assuming each peptide binds an independent site on the target protein.
The direct binding of each peptide to SIP1 was evaluated using the dot-blot binding assay as described elsewhere [37].
Remarkably, the concentration-dependent binding curves obtained for the binding of each peptide to dsDNA displayed significantly different shapes.
All six PlyG peptides (P1-P6) were incubated individually with B. cereus-4342, B. anthracis-Sterne, B. cereus-11778, B. thuringiensis, and B. cereus-10876 cultures and binding of each peptide to the bacteria was detected using Qdot-nanocrystal conjugatedugated with streptavidin.
Similar(56)
Comparing the types of ligands reveals that the binding affinities of each peptide for the two adsorbents are similar.
The binding activity of each peptide was calculated as the fluorescent index (FI), and the FI was determined by: (mean FITC fluorescence with the given peptide − mean FITC fluorescence without peptide) (mean FITC fluorescence without peptide).
The binding affinity of each peptide for lipopolysaccharide (LPS) was determined using dansyl PMB displacement assays (Moore et al. 1986).
We next used dansyl PMB displacement assays to determine the binding affinity of each peptide for LPS.
To further characterize the relative contributions of phosphorylation and farnesylation to binding, the affinity of each peptide for 14-3-3 14-3-3 14-3-3determined by isothermal titration calorimetry.
Using the ImaGene 6.0 (Biodiscovery) image analysis software, the binding of each sarcoid specific peptide with IgGs in each serum was then analyzed and expressed as a ratio of red-to-green fluorescent intensities.
In addition, we compared binding of peptide-free and peptide-loaded DR1 using an equilibrium sandwich ELISA assay (Fig. 4E).
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