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In the later case, the resolution of the electrophoresis would not be sufficient to allow distinction between the binding of each oligonucleotide to the target.
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The Oligowalk program [ 21] included in the software package RNAstructure version 5.3 [ 22] was used to predict the binding affinity of each oligonucleotide to its mRNA target.
Sub-stoichiometric quantities were used in order to maximize any potential contributions from additional protein protein interactions formed upon binding of Alba1 to each oligonucleotide.
Catalysis involves binding of the oligonucleotide to the internal guide sequence of the ribozyme and requires Mg2+ and sequence elements of the catalytic core.
29 Following the binding of the oligonucleotide to the mRNA, degradation by endogenous RNase H is induced.
We observed no binding of 5′SS oligonucleotide by human U1-C protein, which was shown to be properly folded by NMR (Muto et al., 2004).
In these assays we found increased binding of the oligonucleotide using nuclear extracts from polyphenol-treated cells compared with those from DMSO-treated cells (Fig. 6 A).
The binding of an oligonucleotide to the loop of a stem loop structure inserted into the catalytic core of a deoxyribozyme turns its enzymatic activity off, resulting in a NOT gate.
Significant binding of oligonucleotide probes to target DNA and removal of nonspecific binding by membrane washing could both be achieved rapidly, requiring as little as 10 min.
The sensing system is based on adjacent binding of oligonucleotide probes labeled with Renilla luciferase (Rluc) and quantum dot (Qd) on the nucleic acid target.
Watanabe, T. A., Geary, R. S. & Levin, A. A. Plasma protein binding of an antisense oligonucleotide targeting human ICAM-1 (ISIS 2302).
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