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To quantify the binding of each fragment, the dissociation constant Kd was measured by ITC.
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The obtained Dam-fusion/Dam-only ratio reflects the extent of protein binding to each fragment on the microarray, corrected for local differences in chromatin accessibility.
The PCA revealed that egg was present as a protein component of binding of bright fragment of coating (point1).
Substitution of this bulky residue completely abolished the binding of the fragment to AtRaptor1, as in the case of the mammalian TOS motif.
We suggest that the F ab' 2 effect on CD36 expression may partially be the consequence of binding of this fragment of adalimumab to transmembrane TNFα.
For each fragment identified and validated by this methodology, a crystal structure in complex with the humanised MAYM RadA protein was solved to confirm the binding of that fragment in the Phe pocket.
While a full scale screen was not reported using this probe, the authors were able to identify the binding of the fragment N-phenylanthranilic acid to Lck.
For the development of fusion protein, the binding of the target fragment and some kind of endogenous fragment could contribute to the extension of the circulating half-life and help to attenuate the risks of formation of neutralizing antibodies.
We could demonstrate direct binding of a recombinant fragment of C. elegans agrin to purified vertebrate α-DG by a membrane overlay assay.
According to the results, there are no binding of MB3 Fab fragment against rBet v 1.
Fc receptor recognition and binding of the Fc fragment of IgG molecules leads to increased inflammatory signaling [ 25].
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