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The pathway of toxin entry includes 1) binding to a surface receptor - PE binds and enters mammalian cells via binding of domain 1 to the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein (LRP) which is ubiquitously expressed in most tissues and cell types [7].
In contrast, no binding of domain III construct was detected.
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Conformation-specific binding of TARBP2 domain to siRNA led to the formation of homogenous serum-stable complex with targeting potential.
We further investigated the binding of SH2 domain to a phosphorylated peptide from Csk-binding protein upon reduction and oxidation using both NMR and fluorescence approaches.
Similarly, A1-A1 strongly inhibits the binding of dimerized domain V of β2GPI to cardiolipin compared to the monomeric A1 inhibitor.
The binding of SH3 domains to proline-rich ligands is mediated by two conserved hydrophobic pockets within the SH3 domain.
For example, Araujo et al. [13] studied binding of different domains of DNMT1 using solid-state hemimethylated DNA substrates, and in another study DNA binding of various domains of DNMT1 was assessed using unmethylated and hemimethylated oligonucleotides [14].
To map the domains of Sdo1p responsible for the binding and dimerization of the 60S subunit, we further characterized the binding of individual domains of Sdo1p to the 60S subunit.
The solid phase assay was used to monitor the binding of EhCaBP1 domains to G-actin as described earlier [8].
By rosette analysis we found that the binding of 46 SH2 domains was significantly associated with MET phosphorylation (Fig. 6A).
The binding of SH3 domains to proline-rich regions causes the formation of a large number of protein complexes [ 33].
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