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The information resulting from our model systems is distilled into a series of selectivity maps that can be used to read off the relative free energy associated with binding of different ions, and to provide an estimate of the importance of the various factors.
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Many studies have been made of ion-exchange equilibria and the factors that influence the values of K. Different ions are held by exchangers with different strengths.
Here, we demonstrate the use of NMR spectroscopy to characterise binding of ammonium ions to two different enzymes: human histone deacetylase 8 (HDAC8), which is activated allosterically by potassium, and the bacterial Hsp70 homologue DnaK, for which potassium is an integral part of the active site.
Although the preferential binding of copper ions to mammalian PrP has been repeatedly demonstrated, earlier PrP molecules may have harbored different metal ion binding specificities, perhaps reflecting their phylogenetic relationship to zinc transporters.
The growth of NPs proceeds via the binding of S2- ions to Cd2+ ions that are already captured by macromolecules.
The Zn loop serves as the binding site for the Zn ion, while the electrostatic loop stabilizes the binding of both ions.
Different off rates in the presence and absence of Ca2+ have been observed for other C2 domains as well and may arise from cooperative binding of Ca2+ ions and membranes.
The results of the above biological experiments also reveal that the choice of different metal ions has little influence on the DNA binding, DNA cleavage and antimicrobial assay.
In the blood vs lungs comparisons, genes containing high number of TFBs enrich GO processes of ion binding (magnesium ion binding/iron ion binding), transporter activity, metabolic process, as well as ion transmembrane transport.
We study the stochastic binding of single Ca2+ ions to receptors in the cleft using two different models of diffusion: a stochastic and discrete Random Walk (RW) model, and a deterministic continuous model.
The crystal structures and MD simulations presented here provide insight in the coupled binding of three sodium ions and aspartate.
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