As illustrated in Fig. 2, myosin S1 induced α-TM binding to both types of actin, but the numbers of myosin heads required for saturation of truncated and native actins with TM were different.
It is evident that the presence of the more hydrophobic human apoA-I C-terminal amino acids enhances binding to both types of particles.
There did not appear to be any considerable effect of E2 on the ER binding of both cell types.
The other two selected mutations, A114E and D124N, were predicted to slightly improve ΔΔ G of binding while both types of their ΔΔ G of folding were destabilizing.
An S. aureus mutant that was affected in the binding of wall teichoic acids to the peptidoglycan lost the localized binding of both LysM-type murein hydrolases, which resulted in defects in cell shape and increased autolysis (Chan et al. 2013).
The accurate prediction also extends to genes that are identified as being in other ribosomal related functions within "translation", with ROC areas of 0.80, 0.88 and 0.82 in "tRNA binding", "rRNA binding" and "RNA binding" ("RNA binding" is a generalization of both types of RNA), respectively (Figure 1b d).
A similar accuracy was achieved by the prediction of Mg2+ in DNA structures, which were not used for training, revealing that the general mechanism of ion binding by both types of the nucleic acids is sufficiently similar to be captured by a coarse-grained method such as ours.
Scatchard plot analysis of binding curves from both types of equilibrium binding assays revealed a Hill coefficient >1, suggesting cooperativity of ABA binding to LANCL2.
By substituting only the FluB-specific tyrosine at position 6, efficient binding to PA of both types resulted.
In contrast, changing only position 7 of a FluA- to a FluB-specific sequence ablated binding to PA of both types.
