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When either SNAP25 or VAMP2 were pre-exposed to 42°C for 10 min, binding of both proteins was strongly reduced when compared to binding of non-treated proteins (Fig. 5B).
The binding of both proteins was confirmed in plant cells by chromatin immunoprecipitation (ChIP) analysis.
Possibly, genomic binding of both proteins prepares yeast cells to undergo hyphal morphogenesis rapidly in inducing environments.
To provide a functional explanation for the CTCF-UBF interaction, we tested binding of both proteins using chromatin immunoprecipitation (ChIP) in mouse embryonic fibroblasts (MEFs).
We confirm that the binding of both proteins to the membrane is reversible by quantifying the binding affinity of proteins for the membrane.
Moreover, when tested together, we could find no evidence for simultaneous binding of both proteins to the de novo sequence, rather each apparently bound independently.
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Fig. 6D (Supplemental Fig. 6B for all other variants) shows no striking differences in the kinetics of binding of both Protein A- and CD16a to the Fc variants compared to Fc-wt.
Potential nonspecific binding of both protein-coated and uncoated regions was blocked by a final incubation with 1% (w/v) BSA or 1 μg/mL methoxy PEG-NH2 (Nektar Therapeutics, Huntsville, AL) solution.
A comparison of the substrate binding sites of both proteins showed that the alcohol binding site is similar in both proteins and provides ample space for alcohol moieties of substrates.
Both CBP60g and SARD1 were shown to bind to the promoter of SID2 and in EMSA experiments a central DNA binding domain of both proteins was found to bind to an oligomer with the sequence GAAATTTTGG selected from the SID2 promoter [ 19].
With two cholesterol molecules appropriately connected, we hypothesize that the cholesterol binding sites of both proteins will be simultaneously occupied in a manner that will stabilize the protein protein interaction to permit detailed structural analysis of the resulting complex.
More suggestions(15)
binding of both dyes
binding of both factors
binding of both forms
binding of both ligands
binding of both types
binding of both alkaloids
binding of both groups
binding of both components
binding of both mAbs
binding of both receptors
binding of both probes
binding of both linkers
binding of both toxins
binding of both disintegrins
binding of both peptides
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