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As expected, binding of both linkers to the immobilized staples occurred in parallel and in equimolar ratio.
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The Coomassie-stained SDS-PAGE gel and its densitometry quantitation show that efficient binding of linkers occurs only when the length of staples exceeds 20 aa with the 24 aa staple already reaching maximum.
Interestingly, extending the staple to 27 aa compromises binding of the two linkers before the interaction recovers at the 45 aa length.
We show that activities of the potent bivalent inhibitors of thrombin can be reversed by the specific, but much weaker, binding of the linker moiety to protein 'antidotes'antidotes
The binding of a linker histone molecule to the core particle and linker DNA leads to the formation of a new structure, the nucleosome with a variable length of linker DNA [2].
For simplicity, the model does not include binding of cross-linkers to regions flanking the midzone.
Third, the binding of the linker histone H1 to linker DNA greatly affects chromatin folding and condensation.
The binding of the linker histone H1 further compacts the DNA into more condensed chromatin arrays [ 2].
For the CofC4_O5 ligand, additional electron density is present, probably caused by weak binding of the linker and the first DNA base.
Phosphorylation of Crk on Tyr 221 (or CrkL on Tyr 207) causes intramolecular binding of the linker region to the SH2 domain, sequestering the SH2 and SH3N and preventing them from binding target proteins [ 14, 15].
The authors' original observation – that the interdomain linker became sequestered from proteolysis concurrent with a shift to a higher molecular weight complex – led them to a model for BiP oligomerization that is based on binding of the linker of one monomer to the substrate-binding cleft of another monomer, to form a concatenated oligomer.
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