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This formulation assumes that bound virions do not interfere with the binding of additional virions during the period of infection.
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Phagemid rescue using this strain led to markedly improved SA-specific binding of the virions (Fig. 1B, left part).
Based on this, it appears that the binding of p24 to leukocytes (shown in Fig. 3) did not involve binding of infectious virions to CD4.
Next, they test whether CLR01 might block binding of mock virions to fibrils and find that the compound seems to disrupt virions themselves.
Notably, although the compound interferes with an early step during entry, it seems also to enhance the binding of the virions to the cell surface.
Enterovirus infection is initiated by the binding of virions to their respective receptors followed by their internalization into the cells.
An ELISA competitive assay showed that the binding of virions M13-Cry1Ac to CR7-12 waspecificic since it was competed by Cry1Ac toxin (Fig. 2c).
On the other hand, physical binding of virions to the composites could directly inhibit viral contact with host cells since the virus-treated composites were removed from the assay solution prior to infection of the host cells.
Investigations into the signaling processes that are possibly triggered by binding of virions to tetraspanin microdomains are obviously called for.
Because binding of HIV-1 virions or gp120 may also activate STAT1, we used centrifugal filters with 100 kDa molecular weight pores to separate HIV-1 virions and gp120 from lower molecular weight compounds.
However, in view of the possibility of complement-independent and CD4-independent selective binding of virions to erythrocytes, and perhaps to many other cells, with subsequent efficient trans infection of permissive cells, the theoretical possibility arises that CD4 cell-bound infectious virions might be more important for HIV-1 infection in vivo than infection by cell-free virions.
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