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Phosphorylation of Hsp25/27 eliminates its cap-binding activity on barbed ends therefore allowing actin polymerization and remodeling to take place concomitantly with the binding of additional proteins to actin [55].
The observation that two complexes are formed with MftO that contains two MftR sites (yet would be long enough for nonspecific binding of additional proteins) suggests retention of specificity.
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Once bound, TFs mediate the transcriptional output of the enhancer in a number of ways, including interactions with the basal transcriptional machinery at the promoter of the target gene which helps recruit RNA polymerase II (activators) (Kadonaga 2004) or by preventing the binding of additional protein factors at closely located binding sites (short-range repressors) (Small et al. 1991).
The key elements of telomeric chromatin are specific duplex telomere binding proteins that nucleate the assembly of additional proteins.
In addition to the myosins and proteins with CH actin-binding domains, there were a number of additional proteins on the list of proteins that are known to be actin-binding proteins and for which a direct interaction with actin has been shown in the published literature (Table 4).
Certainly, more sensitive IDR predictions would allow for the detection of additional protein binding disordered sites.
A general conclusion that can be drawn from our results with purified components is that H1 can freely diffuse between chromatin binding sites without the contribution of additional protein factors.
The electrostatic repulsion between negatively charged PnA molecules and negatively charged immobilized GM1 could cause a reduction in the adsoprtion rate of additional protein molecules binding to the surface as the PnA surface density increases with increasing bulk concentration.
The resulting local structures trigger long-range RNA conformational rearrangements that enable binding of additional ribosomal proteins (Adilakshmi et al., 2008; Shajani et al., 2011; Kim et al., 2014).
The binding of these additional protein factors leads to condensation of DNA and confers stability to the chromosome.
We find that the ERS1 protein complex, in contrast to the ETR1 protein complex, dynamically changes in response to the ligand ethylene, consistent with the binding of additional transiently-associated protein components.
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