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Mass spectrometry confirmed the monovalent modification of purified USP14 by ISG15VS and excluded covalent binding of additional factors to the complex (Figure 3D).
DNA methylation may affect MYB10 transcription through interference with the RNA-polymerase transcription complex or by preventing binding of additional factors required for transcription.
Alternatively, HS may modulate the binding of additional factors to the complex depending on the presence of HS, or may control the distribution of KAL-1 in the ECM to limit diffusion away from AIY.
In vivo, however, most such binding sites would be non-functional, perhaps because the chromatin conformation around the sites precludes access to the DNA (Segal et al., 2006) or because the target factors require the cooperative binding of additional factors nearby to properly exert their regulatory function (Ravasi et al., 2010).
Possibly, the addition of the bulky EGFP-moiety (which is of similar size as the GTPase itself) interferes with the binding of additional factors needed for recruiting LC3 or for targeting vesicles to LC3 positive compartments, thereby trapping the vesicles at a step just prior to the recruitment of LC3.
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We favor an alternative explanation, in that the LZ3 domain is important for the recruitment of additional factors required for DNA binding in response to stress.
Previous evidence has shown that a significant group of additional factors assist in stress induced binding of HSF1 to the DNA.
We reasoned that the identification of novel telomere-binding proteins would be a first step to the identification of additional factors implicated in telomere biology.
FOXA1 is known as a pioneer factor, a special class of transcription factors that bind to condensed chromatin and facilitate the binding of additional transcription factors [ 22].
FOXA1 has been identified as a "pioneer factor" that binds to chromatin-packaged DNA and opens the chromatin for binding of additional transcription factors, including AR [ 9].
Most FOXA1 studies have focused on its role as a pioneer factor that binds to DNA packaged in chromatin and opens the chromatin for binding of additional transcription factors including AR [ 39, 40].
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