Or, in an alternative model, spreading might be caused by the polymerisation of chromatin factors in a step-wise fashion, for example, a nucleation site of Swi6, bound to H3K9me2, could recruit a histone deacetylase and Clr4, allowing H3K9 methylation of adjacent nucleosomes and binding of additional Swi6 (reviewed by Talbert and Henikoff, 2006).
In addition, the structure shows that binding of one ACE2 to the spike would not sterically hinder binding of additional ACE2 molecules on the other two propeller-blades of each trimer.
The binding of additional C3b to C3 convertases forms C5 convertases, which cleave C5, producing C5a and C5b.
This result suggests that loss of a subfamily 1 receptor may be partially compensated for by the binding of additional CTR1 to the remaining ethylene receptors.
We surmised that these effects might be due to co-operative effects of Coro1C binding to enhance the ability of additional Coro1C to bind F-actin.
Binding of GFP STIM1C∆K and GFP STIM2C∆K is consistent with the presence of additional Ca2+/CaM-binding sites, which are predicted in the CAD of STIM1 and STIM2 [ 20, 30].
These results support the presence of additional HSP72 binding sites on the viral RNP, although these sites are not relevant to functional interactions mediated by the HSP72 binding motif at amino acid position 518-524 of the N protein.
To our surprise, however, pS3291-BRCA2 was barely detectable in the Rad51 immunocomplexes from the soluble fraction, suggesting that an as-yet-unknown mechanism may interfere with Rad51 binding to the pool of endogenous BRCA2 that is physiologically phosphorylated at S3291, despite the presence of additional Rad51-binding BRC motifs in the middle region.
Moreover, the influence of additional RACK1-binding partners on the status of PDE4D5, such as conventional PKC isoforms, e.g. cAMP-activatable PKCα [ 13], is largely unknown and may reveal key areas of novel signalling regulation and cross-talk.
Because a yku80 -135i strain does not exhibit extensive G-tails (Stellwagen et al. 2003), this indicates that the extensive elongation observed in the yku80 -135i strain was not a consequence of additional Cdc13-binding sites.
Molecular modelling and docking studies on TG3 proposed that binding of two additional Ca2+ ions converts the enzyme to the active form on the approach of the first substrate (glutamine-containing polypeptide) (Ahvazi et al. 2004).
